FPR1-Nox1 dependent redox signaling and mucosal wound repair

Generation of Nox1-/-IEC mice Nox1loxp/loxp mice and conditional intestinal epithelial Nox1 knockout mice were generated by crossing Nox1loxp/loxp female mice with mice expressing Cre under the Villin promoter (Jackson Laboratory #004586) to generate VilCre+ Nox1-/female mice (supplemental Figure 4). The construct contained a neomycin selection cassette, as well as LoxP sites flanking exon 13. Thus, the complete exon 13 was replaced with a truncated exon 13 in the intestine by crossing these mice with specific villin Cre mice that express Cre under a villin promoter, providing a tissue specific gene deletion in the intestinal epithelium. The short remaining region of exon 13 becomes fused in-frame with β-galactosidase. The Nox1 gene that encodes a truncated, inactive Nox1 fused to active β-galactosidase is referred herein as VilCre+Nox1-/-. Staining with X-gal demonstrated expression of the transgene in the colon (Figure S4A and 4B).